• Genotyping (SNPs)

• Genomic and Plasmid DNA Isolation

• miRNA and mRNA Profiling

• Primer Efficiency

• Protein Expression Systems

• RNA Isolation

• siRNA

• qRT-PCR*

Molecular Biology

• Antibody Conjugation

• Enzyme Kinetics

• Individual and Complex Immunoprecipitation

• Protein Biotinylation

• Protein Screening and Quantification

• SDS-PAGE and Western Blot

• Whole cell/Cytosolic/Nuclear Lysate Preparation

Protein Chemistry

• Gene Silencing

• Generation of Stably Transfected Cell Lines

• Immortalized Cell Culture

• Immunofluorescence

• Maintenance of Frozen Cell Culture Stocks

• Microscopy

• Plasmid DNA Transfection

• Primary Cell Culture

Cell Culture

• Biocompatibility

• Cell Adhesion (Primary or Tissue Culture)

• Coagulation (Thrombin Generation, TAT, aPTT, PT, etc.)

• Complement Activation (e.g., C3a and 5Cb-9)

• Cytokine Release

• Cytotoxicity

• Drug elution (levels, activity)

• Hemocompatibility (e.g., ASTM 2888-19**)

• Hemolysis (Hgb ELISA)

• Platelet Activation and Adhesion

• Soluble and Cellular Biomarkers*

Device

• Bioenergetics and Mitochondrial Targets

• Cell Adhesion 

• Collagen Gel Contraction

• Halo (thrombolysis) Assay 

• Matrigel Invasion 

• Microfluidic or Parallel Plate Flow Chamber 

• Motility 

• MMPs and TIMPs

• Phenotyping

• Signal Transduction

• Viability, Proliferation, Cytotoxicity, Apoptosis 

Cell Based or Cell Line Assays

• Antibody Isotyping (IgA, IgG, IgE)

• Anti-drug Antibodies

• Assay Development, Qualification, and Validation

• Biosimilars

• Cellular Proteins

• Interference Assays

• Protein Quantification

• Screening Assays

• Signaling Pathway Analysis

• Soluble Biomarkers and Biomarker Panels (e.g., cytokines, GF, CAMS, selectins, IL 1-33, GM-CSF, IFN-g)

ELISA and Multiplex Testing

• Cell Cycle/Proliferation/Apoptosis

• Cell Signaling

• Circulating Endothelial Cells

• Conjugated Antibody Preparation and Detection

• Detection of Cell Surface Proteins

• Immunophenotyping

• Ligand or Antibody Binding; Receptor Occupancy

• Mammalian Cell and Cell-line Antigen/Receptor Analysis

• Microparticles

• Phosphoprotein Detection including VASP*

• Platelet Antigens and Signaling *

• Platelet-Leukocyte Aggregates

• Primary/Secondary Antibody Preparation and Detection

• Reticulocytes

• Whole Blood Cell Analysis (RBC, Platelets, WBC)

Flow Cytometry/Spectral Flow Cytometry

• Activation (CD62(P-selectin), CD63, CD40L, PAC-1)

• Adhesion (Static and Dynamic)

• Agglutination

• Aggregation (PRP* and Whole Blood)

• Angiogenic factors

• Anti-Platelet Antibody Detection

• Clot retraction (Whole Blood and PRP)

• Clot strength (TEG Platelet Mapping*)

• Counts/MPV

• Immature Platelet Function

• Microfluidics

• Non-human ex vivo testing

• Platelet Surface Proteins

• Platelet-Leukocyte Aggregates

• Point-of-Care Testing including VerifyNow

• Procoagulant Function

• Secretion (PF4; Lumi-aggregometry)

• VASP* and other Phosphoproteins

Thrombosis and Hemostasis (Platelets)

• 3-D Tissue Culture 

• Biomarkers (cytokines, chemokine, MMPs, CD Antigens, MPs, Bcl-2, Caspase-3, cleaved PARP, p53, GAPDH, Cytochrome c, PD-1, TRAIL, etc.)

• Biosimilars

• Cancer cell-Platelet Interactions (static and microfluidic assays)

• Cell Adhesion, Migration and Invasion Assays

• Complete Histopathology Analysis (tissue biomarkers) ***

• Epigenetic Study Support (methylated DNA and miRNAs arrays)

• Flow Cytometry (antigen detection, monoclonal antibody binding, ligand occupancy, absolute cell counts, cell signaling pathways, DNA analysis, immunophenotyping,)

• Genetic Variation and Mutation Assays

• Genome-wide Microarrays***

• Immunoprecipitations, SDS/PAGE, Western blot

• Next Generation Sequencing***

• qRT-PCR

• Viral Load Assessment

Oncology

• ADAMTS 13 (levels, activity)

• Coagulopathies 

• Coombs Test

• Erythrocytes: Hemolysis, Complement Activation

• Granulocytes: Antigen Expression (e.g. Mac-1), Oxidative Burst, Platelet-Granulocyte Aggregates

• Hematologic Malignancies (functional and phenotyping)

• Immunogenicity

• Monocytes: CD Antigens Expression, Migration, Platelet-Monocyte Aggregates

• PECAM-1

• Platelet Abnormalities (B-S, GT, Release defect, Storage Pool Disease) and Dysfunction

• Thrombopoietin (TPO)

Hematology

• Activated Clotting Time (ACT)

• Activated Partial Thromboplastin Time (aPTT)

• Antithrombin (ATIII)

• Anti-Xa**

• Coagulation Factor ** (levels/activities)

• D-dimer*

• Fibrinogen**

• Free Tissue factor Pathway Inhibitor (fTFPI)**

• Halo (thrombolysis) Assay 

• t-PA

• PF4*

• Plasmin-Antiplasmin Complex (PAP)**

• Protein C

• Protein S

• Prothrombin Fragment 1.2 (PF1.2*)

• Prothrombin Time (PT)*

• Reversal Agent MOA and Interference Testing

• Russell Viper Venom Test (RVVT)**

• Soluble Thrombomodulin (sTM)**

• Thrombin Activatable Fibrinolytic Inhibitor (TAFI)**

• Thrombin-Antithrombin Complexes (TAT)*

• Thrombin Generation*

• Thromboelastography (TEG Platelet Mapping*) and Fibrinogen*

• b-Thromboglobulin (b-TG)*

• Tissue Factor (TF)

• Tissue Factor Pathway Inhibitor (TFPI) Activity**

• Tissue Plasminogen Activator (tPA)**

• vWF

• WBCT

Coagulation

• BNP, NT-pro-BNP,

• GDF-15, Galectin-3, -9, Copeptin, Cystatin C, sT2

• miRNA

• MR-proANP, MR-proADM

• MMP 1-13

• TIMPs

• CRP

•  TnI, TnT, CKMB

Cardiology and Heart Failure

• BMP

• VEGF

• FGF

• TGF-b

Orthopedic/Bone Biology

• Cell Proliferation, Adhesion, Apoptosis

• Circulating Endothelial Cells

• Homocysteine**

• Hyaluronan**

• L-ficolin**

• LOX-1

• Lp-PLA2

• Microparticles

• CRP

• MCP-1

• S100B

• Proinflammatory biomarker panels

• Receptor and Co-receptors (e.g., CD14)

• Soluble CAMs, selectins, growth factors, cytokine panels

Vascular Bed Diseases; Thrombotic Microangiopathies

* FDA compliant validated tests

** Client-directed validated tests

*** Qualified 3rd party vendor

Key