Lab Testing Services

CirQuest Labs is a cGLP compliant laboratory, State of Tennessee licensed, and CLIA approved. We have utilized our decades of experience in a range of therapeutic and technical areas to deliver spot on data and unparalleled results. 

We have categorized our testing services by technical and therapeutic area. This menu is not a comprehensive list of our capabilities. 

Technical Areas

  • Genotyping (SNPs)

  • Genomic and Plasmid DNA Isolation

  • miRNA and mRNA Profiling

  • Primer Efficiency

  • Protein Expression Systems

  • RNA Isolation

  • siRNA

  • qRT-PCR*

Molecular Biology

  • Antibody Conjugation

  • Enzyme Kinetics

  • Individual and Complex Immunoprecipitation

  • Protein Biotinylation

  • Protein Screening and Quantification

  • SDS-PAGE and Western Blot

  • Whole cell/Cytosolic/Nuclear Lysate Preparation

Protein Chemistry

  • Gene Silencing

  • Generation of Stably Transfected Cell Lines

  • Immortalized Cell Culture

  • Immunofluorescence

  • Maintenance of Frozen Cell Culture Stocks

  • Microscopy

  • Plasmid DNA Transfection

  • Primary Cell Culture

Cell Culture

  • Biocompatibility

  • Cell Adhesion (Primary or Tissue Culture)

  • Coagulation (Thrombin Generation, TAT, aPTT, PT, etc.)

  • Complement Activation (e.g., C3a and 5Cb-9)

  • Cytokine Release

  • Cytotoxicity

  • Drug elution (levels, activity)

  • Hemocompatibility (e.g., ASTM 2888-19**)

  • Hemolysis (Hgb ELISA)

  • Platelet Activation and Adhesion

  • Soluble and Cellular Biomarkers*

Device

  • Bioenergetics and Mitochondrial Targets

  • Cell Adhesion 

  • Collagen Gel Contraction

  • Halo (thrombolysis) Assay 

  • Matrigel Invasion 

  • Microfluidic or Parallel Plate Flow Chamber 

  • Motility 

  • MMPs and TIMPs

  • Phenotyping

  • Signal Transduction

  • Viability, Proliferation, Cytotoxicity, Apoptosis 

Cell Based or Cell Line Assays

  • Antibody Isotyping (IgA, IgG, IgE)

  • Anti-drug Antibodies

  • Assay Development, Qualification, and Validation

  • Biosimilars

  • Cellular Proteins

  • Interference Assays

  • Protein Quantification

  • Screening Assays

  • Signaling Pathway Analysis

  • Soluble Biomarkers and Biomarker Panels (e.g., cytokines, GF, CAMS, selectins, IL 1-33, GM-CSF, IFN-g)

ELISA and Multiplex Testing

  • Cell Cycle/Proliferation/Apoptosis

  • Cell Signaling

  • Circulating Endothelial Cells

  • Conjugated Antibody Preparation and Detection

  • Detection of Cell Surface Proteins

  • Immunophenotyping

  • Ligand or Antibody Binding; Receptor Occupancy

  • Mammalian Cell and Cell-line Antigen/Receptor Analysis

  • Microparticles

  • Phosphoprotein Detection including VASP*

  • Platelet Antigens and Signaling *

  • Platelet-Leukocyte Aggregates

  • Primary/Secondary Antibody Preparation and Detection

  • Reticulocytes

  • Whole Blood Cell Analysis (RBC, Platelets, WBC)

Flow Cytometry/Spectral Flow Cytometry

Therapeutic Areas

 
  • Activation (CD62(P-selectin), CD63, CD40L, PAC-1)

  • Adhesion (Static and Dynamic)

  • Agglutination

  • Aggregation (PRP* and Whole Blood)

  • Angiogenic factors

  • Anti-Platelet Antibody Detection

  • Clot retraction (Whole Blood and PRP)

  • Clot strength (TEG Platelet Mapping*)

  • Counts/MPV

  • Immature Platelet Function

  • Microfluidics

  • Non-human ex vivo testing

  • Platelet Surface Proteins

  • Platelet-Leukocyte Aggregates

  • Point-of-Care Testing including VerifyNow

  • Procoagulant Function

  • Secretion (PF4; Lumi-aggregometry)

  • VASP* and other Phosphoproteins

Thrombosis and Hemostasis (Platelets)

  • 3-D Tissue Culture 

  • Biomarkers (cytokines, chemokine, MMPs, CD Antigens, MPs, Bcl-2, Caspase-3, cleaved PARP, p53, GAPDH, Cytochrome c, PD-1, TRAIL, etc.)

  • Biosimilars

  • Cancer cell-Platelet Interactions (static and microfluidic assays)

  • Cell Adhesion, Migration and Invasion Assays

  • Complete Histopathology Analysis (tissue biomarkers) ***

  • Epigenetic Study Support (methylated DNA and miRNAs arrays)

  • Flow Cytometry (antigen detection, monoclonal antibody binding, ligand occupancy, absolute cell counts, cell signaling pathways, DNA analysis, immunophenotyping,)

  • Genetic Variation and Mutation Assays

  • Genome-wide Microarrays***

  • Immunoprecipitations, SDS/PAGE, Western blot

  • Next Generation Sequencing***

  • qRT-PCR

  • Viral Load Assessment

Oncology

  • ADAMTS 13 (levels, activity)

  • Coagulopathies 

  • Coombs Test

  • Erythrocytes: Hemolysis, Complement Activation

  • Granulocytes: Antigen Expression (e.g. Mac-1), Oxidative Burst, Platelet-Granulocyte Aggregates

  • Hematologic Malignancies (functional and phenotyping)

  • Immunogenicity

  • Monocytes: CD Antigens Expression, Migration, Platelet-Monocyte Aggregates

  • PECAM-1

  • Platelet Abnormalities (B-S, GT, Release defect, Storage Pool Disease) and Dysfunction

  • Thrombopoietin (TPO)

Hematology

  • Activated Clotting Time (ACT)

  • Activated Partial Thromboplastin Time (aPTT)

  • Antithrombin (ATIII)

  • Anti-Xa**

  • Coagulation Factor ** (levels/activities)

  • D-dimer*

  • Fibrinogen**

  • Free Tissue factor Pathway Inhibitor (fTFPI)**

  • Halo (thrombolysis) Assay 

  • t-PA

  • PF4*

  • Plasmin-Antiplasmin Complex (PAP)**

  • Protein C

  • Protein S

  • Prothrombin Fragment 1.2 (PF1.2*)

  • Prothrombin Time (PT)*

  • Reversal Agent MOA and Interference Testing

  • Russell Viper Venom Test (RVVT)**

  • Soluble Thrombomodulin (sTM)**

  • Thrombin Activatable Fibrinolytic Inhibitor (TAFI)**

  • Thrombin-Antithrombin Complexes (TAT)*

  • Thrombin Generation*

  • Thromboelastography (TEG Platelet Mapping*) and Fibrinogen*

  • b-Thromboglobulin (b-TG)*

  • Tissue Factor (TF)

  • Tissue Factor Pathway Inhibitor (TFPI) Activity**

  • Tissue Plasminogen Activator (tPA)**

  • vWF

  • WBCT

Coagulation

  • BNP, NT-pro-BNP,

  • GDF-15, Galectin-3, -9, Copeptin, Cystatin C, sT2

  • miRNA

  • MR-proANP, MR-proADM

  • MMP 1-13

  • TIMPs

  • CRP

  •  TnI, TnT, CKMB

Cardiology and Heart Failure

  • BMP

  • VEGF

  • FGF

  • TGF-b

Orthopedic/Bone Biology

  • Cell Proliferation, Adhesion, Apoptosis

  • Circulating Endothelial Cells

  • Homocysteine**

  • Hyaluronan**

  • L-ficolin**

  • LOX-1

  • Lp-PLA2

  • Microparticles

  • CRP

  • MCP-1

  • S100B

  • Proinflammatory biomarker panels

  • Receptor and Co-receptors (e.g., CD14)

  • Soluble CAMs, selectins, growth factors, cytokine panels

Vascular Bed Diseases; Thrombotic Microangiopathies

* FDA compliant validated tests

** Client-directed validated tests

*** Qualified 3rd party vendor

Key

 
 

Looking for something in particular? Contact us for our complete technical and therapeutic expertise.

Looking for something in particular? Contact us for our complete list of specialized life science services or more info!

CirQuest Labs
140 Collins Street

Memphis, TN 38112
USA

Phone: 901.866.1700

Fax: 901.866.1702

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