Lab Testing Services

CirQuest Labs is a cGLP compliant laboratory, State of Tennessee licensed, and CLIA approved. We have utilized our decades of experience in a range of therapeutic and technical areas to deliver spot on data and unparalleled results. 

We have categorized our testing services by technical and therapeutic area. This menu is not a comprehensive list of our capabilities. 

Technical Areas

  • Genotyping (SNPs)

  • Genomic and Plasmid DNA Isolation

  • miRNA and mRNA Profiling

  • Primer Efficiency

  • Protein Expression Systems

  • RNA Isolation

  • siRNA

  • qRT-PCR*

Molecular Biology

  • Antibody Conjugation

  • Enzyme Kinetics

  • Individual and Complex Immunoprecipitation

  • Protein Biotinylation

  • Protein Screening and Quantification

  • SDS-PAGE and Western Blot

  • Whole cell/Cytosolic/Nuclear Lysate Preparation

Protein Chemistry

  • Gene Silencing

  • Generation of Stably Transfected Cell Lines

  • Immortalized Cell Culture

  • Immunofluorescence

  • Maintenance of Frozen Cell Culture Stocks

  • Microscopy

  • Plasmid DNA Transfection

  • Primary Cell Culture

Cell Culture

  • Biocompatibility

  • Cell Adhesion (Primary or Tissue Culture)

  • Coagulation (Thrombin Generation, TAT, aPTT, PT, etc.)

  • Complement Activation (e.g., C3a and 5Cb-9)

  • Cytokine Release

  • Cytotoxicity

  • Drug elution (levels, activity)

  • Hemocompatibility (e.g., ASTM 2888-19**)

  • Hemolysis (Hgb ELISA)

  • Platelet Activation and Adhesion

  • Soluble and Cellular Biomarkers*

Device

  • Bioenergetics and Mitochondrial Targets

  • Cell Adhesion 

  • Collagen Gel Contraction

  • Halo (thrombolysis) Assay 

  • Matrigel Invasion 

  • Microfluidic or Parallel Plate Flow Chamber 

  • Motility 

  • MMPs and TIMPs

  • Phenotyping

  • Signal Transduction

  • Viability, Proliferation, Cytotoxicity, Apoptosis 

Cell Based or Cell Line Assays

  • Antibody Isotyping (IgA, IgG, IgE)

  • Anti-drug Antibodies

  • Assay Development, Qualification, and Validation

  • Biosimilars

  • Cellular Proteins

  • Interference Assays

  • Protein Quantification

  • Screening Assays

  • Signaling Pathway Analysis

  • Soluble Biomarkers and Biomarker Panels (e.g., cytokines, GF, CAMS, selectins, IL 1-33, GM-CSF, IFN-g)

ELISA and Multiplex Testing

  • Cell Cycle/Proliferation/Apoptosis

  • Cell Signaling

  • Circulating Endothelial Cells

  • Conjugated Antibody Preparation and Detection

  • Detection of Cell Surface Proteins

  • Immunophenotyping

  • Ligand or Antibody Binding; Receptor Occupancy

  • Mammalian Cell and Cell-line Antigen/Receptor Analysis

  • Microparticles

  • Phosphoprotein Detection including VASP*

  • Platelet Antigens and Signaling *

  • Platelet-Leukocyte Aggregates

  • Primary/Secondary Antibody Preparation and Detection

  • Reticulocytes

  • Whole Blood Cell Analysis (RBC, Platelets, WBC)

Flow Cytometry/Spectral Flow Cytometry

Therapeutic Areas

 
  • Activation (CD62(P-selectin), CD63, CD40L, PAC-1)

  • Adhesion (Static and Dynamic)

  • Agglutination

  • Aggregation (PRP* and Whole Blood)

  • Angiogenic factors

  • Anti-Platelet Antibody Detection

  • Clot retraction (Whole Blood and PRP)

  • Clot strength (TEG Platelet Mapping*)

  • Counts/MPV

  • Immature Platelet Function

  • Microfluidics

  • Non-human ex vivo testing

  • Platelet Surface Proteins

  • Platelet-Leukocyte Aggregates

  • Point-of-Care Testing including VerifyNow

  • Procoagulant Function

  • Secretion (PF4; Lumi-aggregometry)

  • VASP* and other Phosphoproteins

Thrombosis and Hemostasis (Platelets)

  • 3-D Tissue Culture 

  • Biomarkers (cytokines, chemokine, MMPs, CD Antigens, MPs, Bcl-2, Caspase-3, cleaved PARP, p53, GAPDH, Cytochrome c, PD-1, TRAIL, etc.)

  • Biosimilars

  • Cancer cell-Platelet Interactions (static and microfluidic assays)

  • Cell Adhesion, Migration and Invasion Assays

  • Complete Histopathology Analysis (tissue biomarkers) ***

  • Epigenetic Study Support (methylated DNA and miRNAs arrays)

  • Flow Cytometry (antigen detection, monoclonal antibody binding, ligand occupancy, absolute cell counts, cell signaling pathways, DNA analysis, immunophenotyping,)

  • Genetic Variation and Mutation Assays

  • Genome-wide Microarrays***

  • Immunoprecipitations, SDS/PAGE, Western blot

  • Next Generation Sequencing***

  • qRT-PCR

  • Viral Load Assessment

Oncology

  • ADAMTS 13 (levels, activity)

  • Coagulopathies 

  • Coombs Test

  • Erythrocytes: Hemolysis, Complement Activation

  • Granulocytes: Antigen Expression (e.g. Mac-1), Oxidative Burst, Platelet-Granulocyte Aggregates

  • Hematologic Malignancies (functional and phenotyping)

  • Immunogenicity

  • Monocytes: CD Antigens Expression, Migration, Platelet-Monocyte Aggregates

  • PECAM-1

  • Platelet Abnormalities (B-S, GT, Release defect, Storage Pool Disease) and Dysfunction

  • Thrombopoietin (TPO)

Hematology

  • Activated Clotting Time (ACT)

  • Activated Partial Thromboplastin Time (aPTT)

  • Antithrombin (ATIII)

  • Anti-Xa**

  • Coagulation Factor ** (levels/activities)

  • D-dimer*

  • Fibrinogen**

  • Free Tissue factor Pathway Inhibitor (fTFPI)**

  • Halo (thrombolysis) Assay 

  • t-PA

  • PF4*

  • Plasmin-Antiplasmin Complex (PAP)**

  • Protein C

  • Protein S

  • Prothrombin Fragment 1.2 (PF1.2*)

  • Prothrombin Time (PT)*

  • Reversal Agent MOA and Interference Testing

  • Russell Viper Venom Test (RVVT)**

  • Soluble Thrombomodulin (sTM)**

  • Thrombin Activatable Fibrinolytic Inhibitor (TAFI)**

  • Thrombin-Antithrombin Complexes (TAT)*

  • Thrombin Generation*

  • Thromboelastography (TEG Platelet Mapping*) and Fibrinogen*

  • b-Thromboglobulin (b-TG)*

  • Tissue Factor (TF)

  • Tissue Factor Pathway Inhibitor (TFPI) Activity**

  • Tissue Plasminogen Activator (tPA)**

  • vWF

  • WBCT

Coagulation

  • BNP, NT-pro-BNP,

  • GDF-15, Galectin-3, -9, Copeptin, Cystatin C, sT2

  • miRNA

  • MR-proANP, MR-proADM

  • MMP 1-13

  • TIMPs

  • CRP

  •  TnI, TnT, CKMB

Cardiology and Heart Failure

  • BMP

  • VEGF

  • FGF

  • TGF-b

Orthopedic/Bone Biology

  • Cell Proliferation, Adhesion, Apoptosis

  • Circulating Endothelial Cells

  • Homocysteine**

  • Hyaluronan**

  • L-ficolin**

  • LOX-1

  • Lp-PLA2

  • Microparticles

  • CRP

  • MCP-1

  • S100B

  • Proinflammatory biomarker panels

  • Receptor and Co-receptors (e.g., CD14)

  • Soluble CAMs, selectins, growth factors, cytokine panels

Vascular Bed Diseases; Thrombotic Microangiopathies

* FDA compliant validated tests

** Client-directed validated tests

*** Qualified 3rd party vendor

Key

 

Looking for something in particular? Contact us for our complete technical and therapeutic expertise.

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CirQuest Labs
140 Collins Street

Memphis, TN 38112
USA

Phone: 901.866.1700

Fax: 901.866.1702

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