Lab Testing Services
CirQuest Labs is a cGLP compliant laboratory, State of Tennessee licensed, and CLIA approved. We have utilized our decades of experience in a range of therapeutic and technical areas to deliver spot on data and unparalleled results.
We have categorized our testing services by technical and therapeutic area. This menu is not a comprehensive list of our capabilities.
Technical Areas
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Genotyping (SNPs)
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Genomic and Plasmid DNA Isolation
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miRNA and mRNA Profiling
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Primer Efficiency
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Protein Expression Systems
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RNA Isolation
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siRNA
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qRT-PCR*
Molecular Biology
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Bioenergetics and Mitochondrial Targets
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Cell Adhesion
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Collagen Gel Contraction
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Halo (thrombolysis) Assay
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Matrigel Invasion
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Microfluidic or Parallel Plate Flow Chamber
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Motility
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MMPs and TIMPs
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Phenotyping
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Signal Transduction
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Viability, Proliferation, Cytotoxicity, Apoptosis
Cell Based or Cell Line Assays
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Antibody Conjugation
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Enzyme Kinetics
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Individual and Complex Immunoprecipitation
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Protein Biotinylation
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Protein Screening and Quantification
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SDS-PAGE and Western Blot
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Whole cell/Cytosolic/Nuclear Lysate Preparation
Protein Chemistry
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Antibody Isotyping (IgA, IgG, IgE)
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Anti-drug Antibodies
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Assay Development, Qualification, and Validation
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Biosimilars
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Cellular Proteins
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Interference Assays
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Protein Quantification
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Screening Assays
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Signaling Pathway Analysis
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Soluble Biomarkers and Biomarker Panels (e.g., cytokines, GF, CAMS, selectins, IL 1-33, GM-CSF, IFN-g)
ELISA and Multiplex Testing
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Gene Silencing
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Generation of Stably Transfected Cell Lines
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Immortalized Cell Culture
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Immunofluorescence
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Maintenance of Frozen Cell Culture Stocks
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Microscopy
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Plasmid DNA Transfection
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Primary Cell Culture
Cell Culture
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Biocompatibility
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Cell Adhesion (Primary or Tissue Culture)
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Coagulation (Thrombin Generation, TAT, aPTT, PT, etc.)
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Complement Activation (e.g., C3a and 5Cb-9)
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Cytokine Release
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Cytotoxicity
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Drug elution (levels, activity)
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Hemocompatibility (e.g., ASTM 2888-19**)
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Hemolysis (Hgb ELISA)
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Platelet Activation and Adhesion
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Soluble and Cellular Biomarkers*
Device
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Cell Cycle/Proliferation/Apoptosis
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Cell Signaling
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Circulating Endothelial Cells
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Conjugated Antibody Preparation and Detection
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Detection of Cell Surface Proteins
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Immunophenotyping
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Ligand or Antibody Binding; Receptor Occupancy
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Mammalian Cell and Cell-line Antigen/Receptor Analysis
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Microparticles
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Phosphoprotein Detection including VASP*
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Platelet Antigens and Signaling *
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Platelet-Leukocyte Aggregates
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Primary/Secondary Antibody Preparation and Detection
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Reticulocytes
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Whole Blood Cell Analysis (RBC, Platelets, WBC)
Flow Cytometry/Spectral Flow Cytometry
Therapeutic Areas
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Activation (CD62(P-selectin), CD63, CD40L, PAC-1)
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Adhesion (Static and Dynamic)
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Agglutination
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Aggregation (PRP* and Whole Blood)
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Angiogenic factors
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Anti-Platelet Antibody Detection
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Clot retraction (Whole Blood and PRP)
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Clot strength (TEG Platelet Mapping*)
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Counts/MPV
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Immature Platelet Function
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Microfluidics
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Non-human ex vivo testing
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Platelet Surface Proteins
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Platelet-Leukocyte Aggregates
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Point-of-Care Testing including VerifyNow
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Procoagulant Function
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Secretion (PF4; Lumi-aggregometry)
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VASP* and other Phosphoproteins
Thrombosis and Hemostasis (Platelets)
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Activated Clotting Time (ACT)
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Activated Partial Thromboplastin Time (aPTT)
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Antithrombin (ATIII)
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Anti-Xa**
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Coagulation Factor ** (levels/activities)
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D-dimer*
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Fibrinogen**
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Free Tissue factor Pathway Inhibitor (fTFPI)**
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Halo (thrombolysis) Assay
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t-PA
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PF4*
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Plasmin-Antiplasmin Complex (PAP)**
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Protein C
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Protein S
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Prothrombin Fragment 1.2 (PF1.2*)
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Prothrombin Time (PT)*
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Reversal Agent MOA and Interference Testing
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Russell Viper Venom Test (RVVT)**
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Soluble Thrombomodulin (sTM)**
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Thrombin Activatable Fibrinolytic Inhibitor (TAFI)**
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Thrombin-Antithrombin Complexes (TAT)*
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Thrombin Generation*
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Thromboelastography (TEG Platelet Mapping*) and Fibrinogen*
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b-Thromboglobulin (b-TG)*
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Tissue Factor (TF)
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Tissue Factor Pathway Inhibitor (TFPI) Activity**
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Tissue Plasminogen Activator (tPA)**
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vWF
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WBCT
Coagulation
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3-D Tissue Culture
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Biomarkers (cytokines, chemokine, MMPs, CD Antigens, MPs, Bcl-2, Caspase-3, cleaved PARP, p53, GAPDH, Cytochrome c, PD-1, TRAIL, etc.)
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Biosimilars
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Cancer cell-Platelet Interactions (static and microfluidic assays)
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Cell Adhesion, Migration and Invasion Assays
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Complete Histopathology Analysis (tissue biomarkers) ***
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Epigenetic Study Support (methylated DNA and miRNAs arrays)
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Flow Cytometry (antigen detection, monoclonal antibody binding, ligand occupancy, absolute cell counts, cell signaling pathways, DNA analysis, immunophenotyping,)
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Genetic Variation and Mutation Assays
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Genome-wide Microarrays***
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Immunoprecipitations, SDS/PAGE, Western blot
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Next Generation Sequencing***
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qRT-PCR
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Viral Load Assessment
Oncology
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ADAMTS 13 (levels, activity)
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Coagulopathies
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Coombs Test
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Erythrocytes: Hemolysis, Complement Activation
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Granulocytes: Antigen Expression (e.g. Mac-1), Oxidative Burst, Platelet-Granulocyte Aggregates
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Hematologic Malignancies (functional and phenotyping)
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Immunogenicity
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Monocytes: CD Antigens Expression, Migration, Platelet-Monocyte Aggregates
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PECAM-1
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Platelet Abnormalities (B-S, GT, Release defect, Storage Pool Disease) and Dysfunction
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Thrombopoietin (TPO)
Hematology
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BNP, NT-pro-BNP,
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GDF-15, Galectin-3, -9, Copeptin, Cystatin C, sT2
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miRNA
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MR-proANP, MR-proADM
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MMP 1-13
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TIMPs
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CRP
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TnI, TnT, CKMB
Cardiology and Heart Failure
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BMP
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VEGF
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FGF
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TGF-b
Orthopedic/Bone Biology
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Cell Proliferation, Adhesion, Apoptosis
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Circulating Endothelial Cells
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Homocysteine**
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Hyaluronan**
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L-ficolin**
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LOX-1
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Lp-PLA2
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Microparticles
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CRP
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MCP-1
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S100B
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Proinflammatory biomarker panels
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Receptor and Co-receptors (e.g., CD14)
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Soluble CAMs, selectins, growth factors, cytokine panels
Vascular Bed Diseases; Thrombotic Microangiopathies
* FDA compliant validated tests
** Client-directed validated tests
*** Qualified 3rd party vendor
Key
Looking for something in particular? Contact us for our complete technical and therapeutic expertise.