Lab Testing Services Menu

CirQuest Labs is a cGLP compliant laboratory, State of Tennessee licensed, and CLIA approved. We have categorized our testing services by technical and therapeutic area. This menu is not a comprehensive list of our capabilities. Please contact us here to learn more.

Technical Areas

Molecular Biology

  • Genotyping

  • Lightcycler Data Collection and Analysis

  • Measurement of Primer Efficiency

  • miRNA Isolation and Analysis

  • Primer/Probe Design

  • qRT-PCR

  • RNA Isolation

  • siRNA

ELISA Testing

  • Cellular Proteins

  • Interference Assays

  • Protein Quantification

  • Signaling Pathway Analysis

  • Soluble Biomarkers

  • Biomarker Development and Validation

Cell Culture (mammalian)

  • Gene Silencing

  • Generation of Stably Transfected Cell Lines

  • Immortalized Cell Culture

  • Maintenance of Frozen Cell Culture Stocks

  • Plasmid DNA Transfection

  • Primary Cell Culture

  • Microscopy

  • Immunoflorescence 

Protein Chemistry

  • Antibody Conjugation

  • Individual and Complex Immunoprecipitation

  • Protein Biotinylation

  • Protein Screening and Quantification

  • SDS-PAGE and Western Blot

  • Whole cell/Cytosolic/Nuclear Lysate Preparation

Flow Cytometry

  • Cell Cycle/Proliferation/Apoptosis

  • Cell Signaling

  • Circulating Endothelial Cells

  • Conjugated Antibody Preparation and Detection

  • Detection of Cell Surface Proteins

  • Signal Transduction

  • Immunophenotyping

  • LISand or Antibody Binding; Receptor Occupancy

  • Mammalian Cell and Cell-line Antigen/Receptor Analysis

  • Microparticles

  • Multi-color Flow Cytometry

  • Phosphoprotein Detection including VASP

  • Platelet Analysis

  • Primary/Secondary Antibody Preparation and Detection

  • Reticulocytes

  • Whole Blood Cell Analysis

Cell-based or Cell line Assays

  • Cell Adhesion 

  • Collagen Gel Contraction 

  • Matrigel Invasion 

  • Microfluidic or Parallel Plate Flow Chamber 

  • Motility 

  • Phenotyping

  • Signal Transduction

  • Viability, Proliferation, Cytotoxicity Apoptosis 

  • Biomarkers

  • MMPs and TIMPs

Device

  • Biocompatibility

  • Blood Cells

  • Cell Lines

  • Platelet Activation and Adhesion

  • ABC hemolysis

  • Cytokine Release

  • Cell Adhesion

  • Soluable and Cellular Biomarker

  • Tissue Culture

Therapeutic Areas

Platelets

  • Activation (CD62, CD63, CD40L, PAC-1)

  • Adhesion (Static and Dynamic)

  • Agglutination

  • Aggregation (PRP and Whole Blood)

  • Clot retraction (Whole Blood and PRP)

  • Procoagulant Function

  • Counts/MPV

  • Immature Platelet Function

  • Microfluidics

  • Platelet Surface Proteins

  • Platelet-Monocyte Aggregates

  • Point-of-Care Testing including VerifyNow

  • Secretion (PF4; Lumiaggregometry)

  • VASP

Hematology

  • Coagulopathies 

  • Erythrocytes: Hemolysis, Complement Activation

  • Granulocytes: Antigen Expression (e.g. Mac-1), Oxidative Burst

  • Monocytes: Antigen Expression, Migration, Platelet-Monocyte Aggregates

  • Platelet Abnormalities 

  • Hematologic Malignancies

  • Immunogenecity

Coagulation

  • Activated Partial Thromboplastin Time (aPTT)

  • Antithrombin III (ATIII)

  • aPTT

  • D-dimer

  • Factor (levels/activities)

  • Fibrinogen

  • Free Tissue factor Pathway Inhibitor (fTFPI)

  • PF4

  • Plasmin-Antiplasmin Complex (PAP)

  • Prothrombin Fragment 1.2 (PF1.2)

  • Prothrombin Time (PT)

  • Russell Viper Venom Test (RVVT)

  • Soluble Thrombomodulin (sTM)

  • Thrombin Activatable Fibrinolytic Inhibitor (TAFI)

  • Thrombin-Antithrombin Complexes (TAT)

  • Tissue Factor Pathway Inhibitor (TFPI)

  • Tissue Plasminogen Activator (tPA)

  • Beta-Thromboglobulin (b-TG)

  • Reversal Agents

Vascular Bed Diseases

  • Biomarkers (e.g., CD62)

  • Circulating Endothelial Cells

  • Microparticles

  • Receptor and Co-receptors (e.g., CD14)

  • Soluble CAMs

  • Pro imflammatory biomarkers

  • Pro liferation, Adhesion, Apoptosis

  • Gene Expression 

Oncology

  • 3D Tissue Culture 

  • Cancer-Platelet Interactions (static and microfluidic assays)

  • Cell Adhesion, Migration and Invasion Assays

  • Complete Histopathology Analysis (tissue biomarkers)

  • Epigenetic Study Support (methylated DNA and microRNAs arrays)

  • Genome-wide Microarrays

  • Immunoprecipitations, SDS/PAGE, Western blot

  • Multiple Array Technologies

  • Next Generation Sequencing

  • qRT-PCR

  • Flow Cytometry (antigen detection, monoclonal antibody binding, ligand occupancy, absolute cell counts, cell signaling pathways, DNA analysis, immunophenotyping)

  • Genetic Variation and Mutation Assays

  • Soluble Biomarkers (cytokines, chemokine, MMPs, MP)

  • Viral Load Assessment

Cardiology and

Heart Failure

  • BNP, NT-pro-BNP,

  • GDF-15, Galectin-3, Copeptin

  • miRNAs such as miR423-5p, miR575 and more.

  • MR-proANP, MR-proADM

  • sST2

  • MMPs

  • TIMPs

© 2019 CirQuest Labs All Rights Reserved

CirQuest Labs
140 Collins Street

Memphis, TN 38112
USA

Phone: 901.866.1700

Fax: 901.866.1702

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